A 2 REPORTER GENE SYSTEM FOR THE ANALYSIS OF BIDIRECTIONAL TRANSCRIPTION FROM THE DIVERGENT MAL6T MAL6S PROMOTER IN SACCHAROMYCES-CEREVISIAE

被引：14

作者：

BELL, PJL

BISSINGER, PH

EVANS, RJ

DAWES, IW

机构：

[1] CRC FOOD IND INNOVAT,SYDNEY,NSW 2052,AUSTRALIA

[2] APPL MICROBIOL,BEAMINSTER,DORSET,ENGLAND

来源：

CURRENT GENETICS | 1995年 / 28卷 / 05期

关键词：

DIVERGENT PROMOTER; MALTOSE; TRANSCRIPTIONAL REPORTER; MEL1;

D O I：

10.1007/BF00310813

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Many sets of genes in Saccharomyces cerevisiae are divergently transcribed, but at present there are no vectors generally available for the simultaneous analysis of divergent transcription from these promoters. In the present study MEL1 and lacZ were used to construct a vector capable of measuring the divergent expression initiated by the MAL6T-MAL6S bi-directional promoter. Our observations demonstrate that the expression of both reporter genes was regulated in a similar fashion to the native MAL6T and MAL6S genes, and that induction was dependent upon the presence of a functional MALR activator gene. The results confirmed that the MAL6T-MAL6S promoter was co-ordinately regulated, repressed by glucose, induced by maltose, and that basal expression was more active in the MAL6S direction than in the MAL6T direction.

引用

页码：441 / 446

页数：6

共 35 条

[1] THE SEQUENCE OF THE DNAS CODING FOR THE MATING-TYPE LOCI OF SACCHAROMYCES-CEREVISIAE [J].

ASTELL, CR ;

AHLSTROMJONASSON, L ;

SMITH, M ;

TATCHELL, K ;

NASMYTH, KA ;

HALL, BD .

CELL, 1981, 27 (01) :15-23

[2]

Barnett J A, 1976, Adv Carbohydr Chem Biochem, V32, P125, DOI 10.1016/S0065-2318(08)60337-6

[3]

BECK CF, 1988, MICROBIOL REV, P318

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] ISOLATION OF 2 DEVELOPMENTALLY REGULATED GENES INVOLVED IN SPORE WALL MATURATION IN SACCHAROMYCES-CEREVISIAE [J].

BRIZA, P ;

BREITENBACH, M ;

ELLINGER, A ;

SEGALL, J .

GENES & DEVELOPMENT, 1990, 4 (10) :1775-1789

[6] MAL63 CODES FOR A POSITIVE REGULATOR OF MALTOSE FERMENTATION IN SACCHAROMYCES-CEREVISIAE [J].

CHANG, YS ;

DUBIN, RA ;

PERKINS, E ;

FORREST, D ;

MICHELS, CA ;

NEEDLEMAN, RB .

CURRENT GENETICS, 1988, 14 (03) :201-209

[7] ORGANIZATION OF THE MAL LOCI OF SACCHAROMYCES - PHYSICAL IDENTIFICATION AND FUNCTIONAL-CHARACTERIZATION OF 3 GENES AT THE MAL6 LOCUS [J].

COHEN, JD ;

GOLDENTHAL, MJ ;

CHOW, T ;

BUCHFERER, B ;

MARMUR, J .

MOLECULAR & GENERAL GENETICS, 1985, 200 (01) :1-8

[8] IDENTIFICATION OF A 2ND TRANS-ACTING GENE CONTROLLING MALTOSE FERMENTATION IN SACCHAROMYCES-CARLSBERGENSIS [J].

DUBIN, RA ;

PERKINS, EL ;

NEEDLEMAN, RB ;

MICHELS, CA .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (08) :2757-2765

[9] CONSTITUTIVE EXPRESSION OF THE MALTOSE FERMENTATIVE ENZYMES IN SACCHAROMYCES-CARLSBERGENSIS IS DEPENDENT UPON THE MUTATIONAL ACTIVATION OF A NONESSENTIAL HOMOLOG OF MAL63 [J].

DUBIN, RA ;

CHARRON, MJ ;

HAUT, SR ;

NEEDLEMAN, RB ;

MICHELS, CA .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (03) :1027-1035

[10] COMPLETE DNA-SEQUENCE OF YEAST CHROMOSOME-XI [J].

DUJON, B ;

ALEXANDRAKI, D ;

ANDRE, B ;

ANSORGE, W ;

BALADRON, V ;

BALLESTA, JPG ;

BANREVI, A ;

BOLLE, PA ;

BOLOTINFUKUHARA, M ;

BOSSIER, P ;

BOU, G ;

BOYER, J ;

BUITRAGO, MJ ;

CHERET, G ;

COLLEAUX, L ;

DALGNANFORNIER, B ;

DELREY, F ;

DION, C ;

DOMDEY, H ;

DUSTERHOFT, A ;

DUSTERHUS, S ;

ENTLAN, KD ;

ERFLE, H ;

ESTEBAN, PF ;

FELDMANN, H ;

FERNANDES, L ;

FOBO, GM ;

FRITZ, C ;

FUKUHARA, H ;

GABEL, C ;

GAILLON, L ;

CARCIACANTALEJO, JM ;

GARCIARAMIREZ, JJ ;

GENT, ME ;

GHAZVINI, M ;

GOFFEAU, A ;

GONZALEZ, A ;

GROTHUES, D ;

GUERREIRO, P ;

HEGEMANN, J ;

HEWITT, N ;

HILGER, F ;

HOLLENBERG, CP ;

HORAITIS, O ;

INDGE, KJ ;

JACQUIER, A ;

JAMES, CM ;

JAUNIAUX, JC ;

JIMENEZ, A ;

KEUCHEL, H .

NATURE, 1994, 369 (6479) :371-378

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