SURAMIN PREVENTS IMPORT OF ACYL-COA OXIDASE INTO RAT-LIVER PEROXISOMES

The inhibitory effect of suramin on the import of [S-35]acyl-CoA oxidase into purified rat liver peroxisomes was investigated in vitro. The import of acyl-CoA oxidase was inhibited completely by 10-mu-M suramin, whilst the latency of catalase remained unchanged. The import value decreased 60% by pretreatment of peroxisomes with 10-mu-M suramin, but it did not decrease by pretreatment of translation products. Polysulfonate compounds which have two clusters of negative charges, such as Cibacron blue F3GA and Trypan blue, as well as suramin, inhibited the import, whilst mono- and disulfonate compounds did not.