ANALYSIS OF FILAMIN AND ALPHA-ACTININ BINDING TO ACTIN BY THE STOPPED-FLOW METHOD

被引：72

作者：

GOLDMANN, WH ^{[1
]}

ISENBERG, G ^{[1
]}

机构：

[1] TECH UNIV MUNICH,DEPT BIOPHYS,D-85747 GARCHING,GERMANY

来源：

FEBS LETTERS | 1993年 / 336卷 / 03期

关键词：

ACTIN; FILAMIN; ALPHA-ACTININ; TRANSIENT KINETICS;

D O I：

10.1016/0014-5793(93)80847-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We ascertained by the stopped flow method the overall association rate constant, k(+1), of filamin and alpha-actinin to fluorescently labelled filamentous actin of similar to 1.3x10(6)M(-1).s(-1) and similar to 1.0x10(6)M(-1).s(-1) as well as the overall dissociation rate constant, k(-1) of similar to 0.6s(-1) and similar to 0.4s(-1), respeetively. The overall equilibrium constant, K, for filamin and alpha-actinin to actin deduced from the relation K=k(+1)/k(-1) agree well with published data.

引用

页码：408 / 410

页数：3

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