CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY WITH KINETOCHORE DETECTION IN COLCHICINE-TREATED HUMAN FIBROBLASTS

被引：28
作者：
ANTOCCIA, A
TANZARELLA, C
MODESTI, D
DEGRASSI, F
机构：
[1] UNIV ROMA LA SAPIENZA,CTR GENET EVOLUZIONIST,CNR,DIPARTIMENTO GENET & BIOL MOLEC,I-00185 ROME,ITALY
[2] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,ROME,ITALY
来源：
MUTATION RESEARCH | 1993年 / 287卷 / 01期
关键词：
MICRONUCLEI; ANEUPLOIDY; ANTIKINETOCHORE STAINING; COLCHICINE; HUMAN FIBROBLASTS;
D O I：
10.1016/0027-5107(93)90148-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A modified micronucleus assay using antikinetochore antibody has been developed in cytokinesis-blocked human fibroblasts as a simple method to identify aneuploidy-inducing agents. Different protocols for inducing binucleated cells by cytochalasin B in colchicine-treated human fibroblasts were investigated. A dose-related increase in kinetochore-positive micronuclei was obtained when cytochalasin B was given subsequent to colchicine treatment. No induction of micronuclei was observed in combined treatments of the two substances. These results indicate that the detection of kinetochores in micronucleated cytokinesis-blocked human fibroblasts can be effectively applied to the identification of environmental agents with aneuploidy-inducing potential. However, in testing such compounds particular attention should be paid to the protocol used for inducing cytokinesis-blocked cells.
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页码：93 / 99
页数：7
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