A STUDY OF THE GENOMIC DIVERSITY OF PLASMODIUM-FALCIPARUM IN SENEGAL .2. TYPING BY THE USE OF THE POLYMERASE CHAIN-REACTION

被引：33

作者：

MERCEREAUPUIJALON, O ^{[1
]}

FANDEUR, T ^{[1
]}

BONNEFOY, S ^{[1
]}

JACQUEMOT, C ^{[1
]}

SARTHOU, JL ^{[1
]}

机构：

[1] INST PASTEUR, UNITE IMMUNOL CELLULAIRE, DAKAR, SOUTH AFRICA

来源：

ACTA TROPICA | 1991年 / 49卷 / 04期

关键词：

PLASMODIUM-FALCIPARUM; ALLELIC POLYMORPHISM; POLYMERASE CHAIN REACTION; STRAIN TYPING; MALARIA VACCINE; MEROZOITE SURFACE ANTIGENS;

D O I：

10.1016/0001-706X(91)90080-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The genomic polymorphism of Plasmodium falciparum was investigated in a series of samples collected in Senegal during one transmission season. PCR analysis was performed on several genes coding for blood-stage antigens: the gene for the major merozoite surface antigen P190, the gene for the second merozoite surface antigen MSA2 and the gene coding for antigen 96tR/GBP130. In each case, several distinct forms of the genes studied were observed. Both the MAD20 and K1 allelic families of P190 genes were observed. PCR analysis of a single variable region did not differentiate each isolate. However, when the data obtained for several markers are combined, each isolate had a specific genotype. Thus, using PCR to study in parallel several loci is a useful tool to genetically type strains.

引用

页码：293 / 304

页数：12

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