Amino acid alterations in Gag that confer the ability to grow in simian cells on HIV-1 are located at a narrow CA region

被引：4
作者：
Nagao, Tamiko [1 ]
Hatcho, Kazuki [1 ]
Doi, Naoya [1 ]
Fujiwara, Sachi [1 ]
Adachi, Akio [1 ]
Nomaguchi, Masako [1 ]
机构：
[1] Univ Tokushima, Inst Hlth Biosci, Dept Virol, Grad Sch, Kuramoto Cho, Tokushima 7708503, Japan
来源：
JOURNAL OF MEDICAL INVESTIGATION | 2009年 / 56卷 / 1-2期
关键词：
HIV-1; Gag; CA; CypA; TRIM5a;
D O I：
10.2152/jmi.56.21
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We previously generated a prototype monkey-tropic human immunodeficiency virus type 1 (HIV-1) designated NL-DT5R. This viral clone has a small region of simian immunodeficiency virus (SIV) within Gag capsid (CA) protein and also SIV Vif protein, but displays a poor growth phenotype in simian cells. To improve the growth potential of NL-DT5R, we have constructed a series of its gag variant viruses. Out of fourteen viral clones generated, five were infectious for simian HSC-F cells, and two of the infectious variants grew similarly with NL-DT5R. Taking their genome structures into consideration, our data here clearly show that a narrow CA region within the Gag protein, i.e., the domain around cyclophilin A (CypA)- binding loop, is critical for the growth ability of HIV-1 in simian cells.
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页码：21 / 25
页数：5
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