A DIRECT RADIOIMMUNOASSAY FOR 5-ALPHA-ANDROSTANE-3-ALPHA,17-BETA-DIOL 17-GLUCURONIDE

被引：9

作者：

RAO, PN ^{[1
]}

RODRIGUEZ, AM ^{[1
]}

MOORE, PH ^{[1
]}

CESSAC, JW ^{[1
]}

机构：

[1] SW FDN BIOMED RES,DEPT ORGAN CHEM,SAN ANTONIO,TX 78228

来源：

STEROIDS | 1992年 / 57卷 / 05期

关键词：

DIRECT RADIOIMMUNOASSAY; ANDROSTANEDIOL-17GLUCURONIDE; TRITIUM AS TRACER;

D O I：

10.1016/0039-128X(92)90105-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis of the 11-alpha-hemiglutaryl-derivative of 5-alpha-androstane-3-alpha, 17-beta-diol 17-glucuronide (androstanediol-17G) starting from androsta-4,9(11)-diene-3,17-dione through a 10-step sequence and the preparation of its bovine serum albumin conjugate is described. By using this conjugate, antiserum was raised in rabbits which proved to be very specific for androstanediol-17G. A direct radioimmunoassay using a double antibody procedure is described for the measurement of androstanediol-17G from plasma without prior chromatography.

引用

页码：216 / 221

页数：6