SYNTHESIS OF AFFINITY-CHROMATOGRAPHY AND ELECTROPHORESIS MATRICES FOR THE PURIFICATION OF BILE-ACID TRANSPORT PROTEINS

被引：0
作者：
MULLNER, S
MOLLER, W
ZIEGLER, K
FASOLD, H
机构：
[1] UNIV FRANKFURT KLINIKUM,INST BIOCHEM,W-6000 FRANKFURT 70,GERMANY
[2] UNIV GIESSEN,FACHBEREICH 18,INST PHARMAKOL & TOXIKOL,W-6300 GIESSEN,GERMANY
来源：
CHROMATOGRAPHIA | 1991年 / 32卷 / 5-6期
关键词：
AFFINITY CHROMATOGRAPHY; AFFINITY ELECTROPHORESIS; BILE ACID TRANSPORT PROTEINS; BILE ACID DERIVATIVES;
D O I：
10.1007/BF02276251
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
For the purification of the bile acid transport proteins from rat hepatocytes high affinity taurocholic acid derivatives were synthesized. One type was designed for coupling to a Sepharose matrix and the other for copolymerization with acrylamide. The resulting matrices were found to be suitable for affinity chromatography and affinity electrophoresis. In both syntheses, 3-alpha, 12-alpha-dihydroxy-7-beta-amino (tauro) cholic acid derivatives were used as the starting material, since substitution in this position had previously been shown not to alter transport affinity constant.
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页码：265 / 268
页数：4
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