SYNTHESIS OF AFFINITY-CHROMATOGRAPHY AND ELECTROPHORESIS MATRICES FOR THE PURIFICATION OF BILE-ACID TRANSPORT PROTEINS

被引：0

作者：

MULLNER, S

MOLLER, W

ZIEGLER, K

FASOLD, H

机构：

[1] UNIV FRANKFURT KLINIKUM,INST BIOCHEM,W-6000 FRANKFURT 70,GERMANY

[2] UNIV GIESSEN,FACHBEREICH 18,INST PHARMAKOL & TOXIKOL,W-6300 GIESSEN,GERMANY

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 5-6期

关键词：

AFFINITY CHROMATOGRAPHY; AFFINITY ELECTROPHORESIS; BILE ACID TRANSPORT PROTEINS; BILE ACID DERIVATIVES;

D O I：

10.1007/BF02276251

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For the purification of the bile acid transport proteins from rat hepatocytes high affinity taurocholic acid derivatives were synthesized. One type was designed for coupling to a Sepharose matrix and the other for copolymerization with acrylamide. The resulting matrices were found to be suitable for affinity chromatography and affinity electrophoresis. In both syntheses, 3-alpha, 12-alpha-dihydroxy-7-beta-amino (tauro) cholic acid derivatives were used as the starting material, since substitution in this position had previously been shown not to alter transport affinity constant.

引用

页码：265 / 268

页数：4

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