THE STRUCTURE OF SPINACH PHOTOSYSTEM-I STUDIED BY ELECTRON-MICROSCOPY

被引：72

作者：

BOEKEMA, EJ ^{[1
]}

WYNN, RM ^{[1
]}

MALKIN, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT PLANT BIOL, BERKELEY, CA 94720 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1017卷 / 01期

基金：

美国国家科学基金会;

关键词：

(Spinach); Electron microscopy; Image analysis; Photosystem I structure;

D O I：

10.1016/0005-2728(90)90177-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex. © 1990.

引用

页码：49 / 56

页数：8

共 33 条

[1] SEPARATION AND CHARACTERIZATION OF STROMA AND GRANA MEMBRANES - EVIDENCE FOR HETEROGENEITY IN ANTENNA SIZE OF BOTH PHOTOSYSTEM-I AND PHOTOSYSTEM-II [J].

ANDREASSON, E ;

SVENSSON, P ;

WEIBULL, C ;

ALBERTSSON, PA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 936 (03) :339-350

[2] SPATIAL RELATIONSHIP OF PHOTOSYSTEM-I, PHOTOSYSTEM-II, AND LIGHT-HARVESTING COMPLEX IN CHLOROPLAST MEMBRANES [J].

ARMOND, PA ;

STAEHELIN, LA ;

ARNTZEN, CJ .

JOURNAL OF CELL BIOLOGY, 1977, 73 (02) :400-418

[3] COMPARISON OF 4X6-MERIC HEMOCYANINS FROM 3 DIFFERENT ARTHROPODS USING COMPUTER ALIGNMENT AND CORRESPONDENCE-ANALYSIS [J].

BIJLHOLT, MMC ;

VANHEEL, MG ;

VANBRUGGEN, EFJ .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 161 (01) :139-153

[4] STRUCTURE OF MITOCHONDRIAL F1-ATPASE STUDIED BY ELECTRON-MICROSCOPY AND IMAGE-PROCESSING [J].

BOEKEMA, EJ ;

BERDEN, JA ;

VANHEEL, MG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 851 (03) :353-360

[5] REFINED ANALYSIS OF THE TRIMERIC STRUCTURE OF THE ISOLATED PHOTOSYSTEM-I COMPLEX FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP [J].

BOEKEMA, EJ ;

DEKKER, JP ;

ROGNER, M ;

WITT, I ;

WITT, HT ;

VANHEEL, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 974 (01) :81-87

[6] EVIDENCE FOR A TRIMERIC ORGANIZATION OF THE PHOTOSYSTEM-I COMPLEX FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP [J].

BOEKEMA, EJ ;

DEKKER, JP ;

VANHEEL, MG ;

ROGNER, M ;

SAENGER, W ;

WITT, I ;

WITT, HT .

FEBS LETTERS, 1987, 217 (02) :283-286

[7] ISOLATION OF PHOTOSYSTEM-I COMPLEXES FROM OCTYL GLUCOSIDE SODIUM DODECYL-SULFATE SOLUBILIZED SPINACH THYLAKOIDS - CHARACTERIZATION AND RECONSTITUTION INTO LIPOSOMES [J].

DUNAHAY, TG ;

STAEHELIN, LA .

PLANT PHYSIOLOGY, 1985, 78 (03) :606-613

[8]

FISH LE, 1986, J BIOL CHEM, V261, P8134

[9]

FISH LE, 1985, J BIOL CHEM, V260, P1413

[10] INVESTIGATION OF THE STRUCTURE OF TRIMERIC AND MONOMERIC PHOTOSYSTEM-I REACTION CENTER COMPLEXES [J].

FORD, RC ;

HOLZENBURG, A .

EMBO JOURNAL, 1988, 7 (08) :2287-2293

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