GUANYLATE-CYCLASE IN OLFACTORY CILIA FROM RAT AND PIG

被引：12

作者：

STEINLEN, S ^{[1
]}

KLUMPP, S ^{[1
]}

SCHULTZ, JE ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST PHARMAZEUT,MORGENSTELLE 8,W-7400 TUBINGEN 1,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1054卷 / 01期

关键词：

Cilium; Guanylate cyclase; Olfaction; Trachea;

D O I：

10.1016/0167-4889(90)90206-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A guanylate cyclase was identified in cilia from rat and pig olfactory epithelia. Enzyme activities were 200-250 and 90-100 pmol/min · mg-1, respectively. Activity required the presence of non-ionic detergents, e.g., 0.1% Lubrol PX. MnGTP, not MgGTP was used as a substrate. Furthermore, 0.9 mM free Mn2+ was necessary for optimal activity indicating a regulatory site for a divalent cation. The guanylate cyclase displayed sigmoidal Michaelis-Menten kinetics suggesting cooperativity between MnGTP and enzyme. S0.5 was 160 μM MnGTP. The Hill coefficient of 1.7 indicates that more than one class of substrate-binding sites interact in a positive cooperative manner. ATP inhibited the enzyme and linearized plots of substrate kinetics with MnGTP. SH-Blocking agents reversibly inhibited enzyme activity. Sodium azide and nitroprusside were without effect as were several odorants. A guanylate cyclase activity in cilia from tracheal tissue had properties similar to the olfactory enzyme. © 1990.

引用

页码：69 / 72

页数：4

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