IS POLYAMINE DECREASE A COMMON FEATURE OF APOPTOSIS - EVIDENCE FROM GAMMA-RAYS-INDUCED AND HEAT-SHOCK-INDUCED CELL-DEATH

被引：53

作者：

GRASSILLI, E ^{[1
]}

DESIDERIO, MA ^{[1
]}

BELLESIA, E ^{[1
]}

SALOMONI, P ^{[1
]}

BENATTI, F ^{[1
]}

FRANCESCHI, C ^{[1
]}

机构：

[1] UNIV MILAN,CNR,CTR RES CELL PATHOL,I-20133 MILAN,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 216卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report that in rat thymocytes undergoing apoptosis upon two different stimuli, such as heat shock treatment and gamma irradiation, an early mRNA accumulation of ornithine decarboxylase (ODC) - the rate-limiting enzyme of polyamine biosynthesis - was followed by a very marked increase in ODC activity (28-40 and 6-8-fold, respectively). However, polyamine levels started to decrease before the appearance of DNA laddering, being putrescine and spermidine strongly diminished (8-12 ha), and spermine even depleted (12 hs). Taken together with our previous data on another model of apoptosis, i.e., glucocorticoid-induced cell death (Desiderio et al., Cell Growth Differ. 6: 505-513, 1995), these results suggest that an imbalance of polyamine metabolism. i.e., a strong activation of ODC and a paradoxical decrease of the intracellular polyamine content, might be a general feature of the apoptotic process. (C) 1995 Academic Press, Inc.

引用

页码：708 / 714

页数：7

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