TRANSFORMATION OF ASPERGILLUS-ORYZAE WITH A DOMINANT SELECTABLE MARKER

被引：8

作者：

CHEEVADHANARAK, S ^{[1
]}

SAUNDERS, G ^{[1
]}

RENNO, DV ^{[1
]}

FLEGEL, TW ^{[1
]}

HOLT, G ^{[1
]}

机构：

[1] POLYTECH CENT LONDON, SCH BIOL & HLTH SCI, 115 NEW CAVENDISH ST, LONDON W1M 8JS, ENGLAND

来源：

JOURNAL OF BIOTECHNOLOGY | 1991年 / 19卷 / 01期

关键词：

ASPERGILLUS-ORYZAE; PHLEOMYCIN RESISTANCE; TRANSFORMATION;

D O I：

10.1016/0168-1656(91)90079-B

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200-mu-g ml-1 phleomycin. A frequency of 3 to 7 transformants per 10(6) viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites.

引用

页码：117 / 122

页数：6

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