PROBING THE SECONDARY QUINONE (QB) ENVIRONMENT IN PHOTOSYNTHETIC BACTERIAL REACTION CENTERS BY LIGHT-INDUCED FTIR DIFFERENCE SPECTROSCOPY

被引：63
作者：
BRETON, J
BERTHOMIEU, C
THIBODEAU, DL
NABEDRYK, E
机构：
[1] DBCM, SBE, C.E.N. Saclay
来源：
FEBS LETTERS | 1991年 / 288卷 / 1-2期
关键词：
FOURIER TRANSFORM INFRARED SPECTROSCOPY; BACTERIAL REACTION CENTER; PHOTOSYNTHESIS; PRIMARY QUINONE; SECONDARY QUINONE; UBIQUINONE;
D O I：
10.1016/0014-5793(91)81014-Y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The photoreduction of the secondary electron acceptor, Q(B), has been characterized by light-induced Fourier transform infrared difference spectroscopy of Rb. sphaeroides and Rp. viridis reaction centers. The reaction centers were supplemented with ubiquinone (UQ10 or UQ0). The Q(B)-state was generated either by continuous illumination at very low intensity or by single flash in the presence of redox compounds which rapidly reduce the photooxidized primary electron donor P+. This approach yields spectra free from P and P+ contributions making possible the study of the microenvironment of Q(B) and Q(B)-. Assignments are proposed for the C...O vibration of Q(B)- and tentatively for the C = O and C = C vibrations of Q(B).
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页码：109 / 113
页数：5
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