DEVELOPMENT OF CHICKEN ANTIBODIES TOWARD THE HUMAN THYROTROPIN RECEPTOR PEPTIDES AND THEIR BIOACTIVITIES

被引：51

作者：

OHMORI, M

ENDO, T

ONAYA, T

机构：

[1] The Third Department of Internal Medicine, University of Yamanashi Medical School, 409-38, Tamaho

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90534-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have synthesized four peptides (P2, P4, E3 and P1) corresponding to different segments of human thyrotropin (TSH) receptor. We have obtained antibodies by immunizing them to chickens, and antibodies are evaluated for their thyroid stimulating antibody (TSAb), thyroid stimulation blocking antibody (TSBAb) and TSH-binding inhibitor immunoglobulin (TBII) activities. None of the antibodies had TSAb activity. Antibodies against human TSH receptor specific region such as P2 and P4 (P2: No. 372-397, P4: No. 341-358) had TSBAb and TBII activities. Anti-E3 antibody (E3: the third putative extracellular loop, No. 649-661) had only TSBAb activity. Anti-P1 antibody (P1: high homology with pig LH CG receptor, No. 398-417), however, had none. These results suggest that anti-TSH receptor antibodies to different antigenic epitopes show heterogeneity in their biological activities. © 1991.

引用

收藏

页码：399 / 403

页数：5

相关论文

共 11 条

[1] INVITRO CONVERSION OF BLOCKING TYPE ANTI-TSH RECEPTOR ANTIBODY TO THE STIMULATING TYPE BY ANTI-HUMAN IGG ANTIBODIES [J].

AMINO, N ;

WATANABE, Y ;

TAMAKI, H ;

IWATANI, Y ;

MIYAI, K .

CLINICAL ENDOCRINOLOGY, 1987, 27 (05) :615-624

[2] A SENSITIVE AND PRACTICAL ASSAY FOR THYROID-STIMULATING ANTIBODIES USING FRTL-5 THYROID-CELLS [J].

KASAGI, K ;

KONISHI, J ;

IIDA, Y ;

TOKUDA, Y ;

ARAI, K ;

ENDO, K ;

TORIZUKA, K .

ACTA ENDOCRINOLOGICA, 1987, 115 (01) :30-36

[3] CLONING, SEQUENCING AND EXPRESSION OF THE HUMAN THYROTROPIN (TSH) RECEPTOR - EVIDENCE FOR BINDING OF AUTOANTIBODIES [J].

LIBERT, F ;

LEFORT, A ;

GERARD, C ;

PARMENTIER, M ;

PERRET, J ;

LUDGATE, M ;

DUMONT, JE ;

VASSART, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1250-1255

[4] CLONING, SEQUENCING AND EXPRESSION OF HUMAN TSH RECEPTOR [J].

MISRAHI, M ;

LOOSFELT, H ;

ATGER, M ;

SAR, S ;

GUIOCHONMANTEL, A ;

MILGROM, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (01) :394-403

[5] SENSITIVE VISUALIZATION OF ANTIGEN-ANTIBODY REACTIONS IN DOT AND BLOT IMMUNE OVERLAY ASSAYS WITH IMMUNOGOLD AND IMMUNOGOLD SILVER STAINING [J].

MOEREMANS, M ;

DANEELS, G ;

VANDIJCK, A ;

LANGANGER, G ;

DEMEY, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 74 (02) :353-360

[6] MOLECULAR-CLONING, SEQUENCE AND FUNCTIONAL EXPRESSION OF THE CDNA FOR THE HUMAN THYROTROPIN RECEPTOR [J].

NAGAYAMA, Y ;

KAUFMAN, KD ;

SETO, P ;

RAPOPORT, B .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1184-1190

[7] AN IMPROVED RADIORECEPTOR ASSAY FOR TSH RECEPTOR ANTIBODIES [J].

SHEWRING, G ;

SMITH, BR .

CLINICAL ENDOCRINOLOGY, 1982, 17 (04) :409-417

[8] AUTOANTIBODIES TO THE THYROTROPIN RECEPTOR [J].

SMITH, BR ;

MCLACHLAN, SM ;

FURMANIAK, J .

ENDOCRINE REVIEWS, 1988, 9 (01) :106-121

[9] MONOCLONAL-ANTIBODIES TO THE THYROTROPIN RECEPTOR - STIMULATING AND BLOCKING ANTIBODIES DERIVED FROM THE LYMPHOCYTES OF PATIENTS WITH GRAVES-DISEASE [J].

VALENTE, WA ;

VITTI, P ;

YAVIN, Z ;

YAVIN, E ;

ROTELLA, CM ;

GROLLMAN, EF ;

TOCCAFONDI, RS ;

KOHN, LD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21) :6680-6684

[10] AN INSERTION IN THE HUMAN THYROTROPIN RECEPTOR CRITICAL FOR HIGH-AFFINITY HORMONE BINDING [J].

WADSWORTH, HL ;

CHAZENBALK, GD ;

NAGAYAMA, Y ;

RUSSO, D ;

RAPOPORT, B .

SCIENCE, 1990, 249 (4975) :1423-1425