GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF KYLLINGA TRICEPS

Objective: To isolate and analyze the chemical components in crude methanolic extract from the South Indian medicinal plant of Kyllinga triceps by gas chromatography-mass spectrometry (GC-MS). Methods: Preliminary phytochemical screening of the extract was carried out according to the standard method described by Brindha et al. The shade dried powder was extracted with methanol by using soxhlet extractor. The GC-MS analysis of methanolic extract of Kyllinga triceps was performed using a GC-MS equipment Thermo GC-TRACE ultra ver., 5.0, Thermo MS DSQ II. Experimental conditions of GC-MS system were as follows: TR5- MS capillary standard non-polar column, dimension: 30 Mts, ID: 0.25 mm, Film: 0.25 mu m was used and flow rate of mobile phase (carrier gas: He) was set at 1.0 ml/min. Crude samples which dissolved in methanol were run fully at a range of 50-650 m/z and the results were compared by using Wiley Spectral library search programme. Results: The phytochemical analysis of the extract revealed the presence of steroids, alkaloids, sugar, phenolic compounds, flavonoids, saponins, tannins and amino acids. The GC-MS analysis provided different peaks determining the presence of twenty two different phytocompounds namely Methanone, (1-hydroxycyclohexyl) phenyl - (14.88%), Tricyclo [4.2.1.1 ( 2,5)] dec-7-en-9-ol-(6.62%), n-deutero-3-ethyl-3-phenyl-2,6-dioxopiperidine-(5.19%), 2-Propenoic acid, 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl ester, exo-(4.08 %), (E)-2-ethylidene-3-oxo-GA9 16a, 17-epoxide methyl ester (2.94%), Z)-4-chloro-2-methyl-1-phenylbut-3-en-1-one (2.05 %), etc. Conclusion: The presence of various bioactive compounds confirms the application of Kyllinga triceps for various ailments. However, isolation of individual phytochemical constituents may be proceeded to find out a novel drug.