AN AMPEROMETRIC ALGAL BIOSENSOR FOR HERBICIDE DETECTION EMPLOYING A CARBON CATHODE OXYGEN-ELECTRODE

被引：23
作者：
PANDARD, P
RAWSON, DM
机构：
[1] Applied Research and Consultancy Centre, Luton College of Higher Education, Luton, Bedfordshire, LU2 8LE, Hitchin Road
[2] Laboratoire de Toxicologie, Centre des Sciences de l'Environment, Metz, 57000
来源：
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1993年 / 8卷 / 03期
关键词：
D O I：
10.1002/tox.2530080309
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A method is described for continuous monitoring of the photosynthetic oxygen evolution of the microalga Chlorella vulgaris, which was immobilized between alumina filters on the surface of a teflon-covered carbon electrode. Sensitivity of the algal biosensor to herbicides was tested using an electrochemical flow cell with illumination provided by light-emitting diodes. This configuration also allows the biosensor to be used in real-time environmental monitoring. The herbicides atrazine, isoproturon, and propanil were detected within 30 min at concentrations below 100 mug dm-3, while the herbicide bromoxynil was only detected after several hours at mg dm-3, concentrations. Good sensor stability was obtained in the absence of pollutants, with more than 24 h of continuous monitoring being achieved. (C) 1993 John Wiley & Sons, Inc.
引用
收藏
页码：323 / 333
页数：11
相关论文
共 23 条
  • [1] (1980)
  • [2] Bitton G., Dutton R.J., Koopman B., Cell permeability to toxicants: An important parameter in toxicity tests using bacteria, Critical Reviews in Environmental Control, 18, pp. 177-188, (1988)
  • [3] Bulich A.A., Greene M.W., Use of luminescent bacteria for biological monitoring of water quality, International Symposium on Analytical Applications of Bioluminescence and Chemiluminescence, pp. 193-211, (1979)
  • [4] Clark L.C., Monitoring and control of blood and tissue oxygen tensions, Trans. Am. Soc. Artific. Intern. Organs, 2, pp. 41-48, (1956)
  • [5] Corbett J.R., Wright K., Baillie A.C., Herbicides interfering with photosynthesis, The Biochemical Mode of Action of Pesticides, pp. 50-98, (1984)
  • [6] Dorward E.J., Barisas B.G., Acute toxicity screening of water pollutants using a bacterial electrode, Environ. Sci. Technol., 18, pp. 967-972, (1984)
  • [7] Dutka B.J., Nyholm N., Petersen J., Comparison of several microbiological toxicity screening tests, Water Res., 17, pp. 1363-1368, (1983)
  • [8] Hyeon S.-B., Nishida M., Ohsaka A., Kim J.-M., Suzuki A., A simple bioassay for chemicals active to the photosynthetic or respiratory systems of plants, Agricultural and Biological Chemistry, 46, pp. 811-812, (1982)
  • [9] Karube I., Tamiya E., Biosensors for environmental control, Pure and Applied Chemistry, 59, pp. 545-554, (1987)
  • [10] Kratky B.A., Warren G.F., The use of three simple, rapid bioassays on forty‐two herbicides, Weed Res., 11, pp. 257-262, (1971)
123