VIBRIOPHAGE N4 DNA IS NONPERMUTED AND TERMINALLY REDUNDANT

被引：2
作者：
GHOSH, AN
CHAKRABARTI, BK
CHATTOPADHYAY, DJ
SIL, S
机构：
[1] UNIV COLL SCI CALCUTTA,DEPT BIOCHEM,CALCUTTA 700019,W BENGAL,INDIA
[2] UNIV COLL SCI,DEPT BIOPHYS & MOLEC BIOL,CALCUTTA 700009,W BENGAL,INDIA
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 1995年 / 41卷 / 09期
关键词：
VIBRIOPHAGE N4; DNA; VIBRIO CHOLERAE; DENATURATION MAPPING;
D O I：
10.1139/m95-115
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phage N4 is one of the five newly isolated phages used in a new phage typing scheme for Vibrio cholerae O1 biovar EITor strains and belongs to the Podoviridae family. Electron microscopic studies showed that the phage N4 has a DNA of 40.4 +/- 0.1 kb. A partial denaturation map of the N4 DNA has been constructed. It has been shown with the help of this partial denaturation map that phage N4 genome is nonpermuted. Circularization of the phage genome on treatment with exonuclease In, followed by incubation in 50% formamide, indicates a terminal redundancy.
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收藏
页码：842 / 845
页数：4
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