MOBILIZATION AND LOCATION OF THE GENETIC DETERMINANT OF CHLORAMPHENICOL RESISTANCE FROM LACTOBACILLUS-PLANTARUM CATC2R

被引：51

作者：

AHN, C ^{[1
]}

COLLINSTHOMPSON, D ^{[1
]}

DUNCAN, C ^{[1
]}

STILES, ME ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

PLASMID | 1992年 / 27卷 / 03期

关键词：

D O I：

10.1016/0147-619X(92)90018-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mobilization of a nonconjugative plasmid (pCaT) that mediates chloramphenicol resistance inLactobacillus plantarum caTC2R was achieved by comobilization with the conjugative plasmid pAMβ1. The conjugation studies confirmed that the 8.5-kb pCaT inL. plantarum caTC2R contains the gene responsible for chloramphenicol resistance and that the plasmid has several unique restriction sites which make it useful for genetic studies inCarnobacterium spp. Cloning studies showed that the gene responsible for chloramphenicol resistance is located in the 2.6-kb EcoRV-SalI region of pCaT. This was confirmed by probing the 3.0-kb BglII fragment of pCaT with a biotin-labeled 1.6-kb BstEII-HpaII fragment from the streptococcal-derived plasmid pVA797(Cmr). Expression of chloramphenicol resistance inCarnobacterium as well as in otherLactobacillus species was achieved by electrotransformation using donor DNA from pCaT. © 1992 Academic Press, Inc. All rights of reproduction in any form reserved.

引用

页码：169 / 176

页数：8

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