DETERMINATION OF VITAMIN-K IN SERUM USING HPLC WITH POSTCOLUMN REACTION AND FLUORESCENCE DETECTION

被引：30

作者：

LAMBERT, WE ^{[1
]}

DELEENHEER, AP ^{[1
]}

LEFEVERE, MF ^{[1
]}

机构：

[1] STATE UNIV GHENT, FAC FARMACEUT WETENSCHAPPEN, MED BIOCHEM & KLIN ANAL LAB, B-9000 GHENT, BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1986年 / 24卷 / 02期

关键词：

D O I：

10.1093/chromsci/24.2.76

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, sensitive and selective detection method for vitamin K1 in human serum is reported. After the chromatographic separation, vitamin K is converted to its hydroquinone form in a wet-chemical post-column reduction reaction with tetramethylammonium octahydridotriborate. The reaction proceeds in an open tubular knitted reaction coil at elevated temperature. This system, when combined with a two-step multidimensional liquid chromatographic separation, has proven useful for the quantitative determination of serum concentrations of vitamin K1 in normal, healthy individuals.

引用

页码：76 / 79

页数：4

共 15 条

[1] FLUOROMETRIC-DETERMINATION OF PHYLLOQUINONE AND MENAQUINONE-4 IN BIOLOGICAL-MATERIALS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].