COMPARISON OF MTT COLORIMETRIC ASSAY AND TRITIATED-THYMIDINE UPTAKE FOR LYMPHOCYTE-PROLIFERATION ASSAYS USING CHICKEN SPLENOCYTES

被引：152

作者：

BOUNOUS, DI ^{[1
]}

CAMPAGNOLI, RP ^{[1
]}

BROWN, J ^{[1
]}

机构：

[1] UNIV GEORGIA,COLL VET MED,DEPT MED MICROBIOL,ATHENS,GA 30602

来源：

AVIAN DISEASES | 1992年 / 36卷 / 04期

关键词：

D O I：

10.2307/1591566

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) colorimetric assay was compared with the conventional tritiated thymidine deoxyriboside (H-3-TdR) incorporation for assay of lymphocyte blastogenesis using mononuclear cells isolated from the spleens of specific-pathogen-free chickens. The study was undertaken in an effort to simplify methods for assessing avian lymphocyte proliferation, specifically for evaluating response to mitogens or for indirect measurement of T-cell growth factors. The results from stimulated cells in both assay methods were significantly different from results from the control cells, and the MTT assay results regressed in a significant linear manner on counts from H-3-TdR incorporation. On this basis, the MTT assay is a valid test for evaluation of lymphocyte proliferation of chicken splenocytes.

引用

页码：1022 / 1027

页数：6

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