USING RANDOMLY AMPLIFIED POLYMORPHIC DNA FOR EVALUATING GENETIC-RELATIONSHIPS AMONG PAPAYA CULTIVARS

被引：125

作者：

STILES, JI ^{[1
]}

LEMME, C ^{[1
]}

SONDUR, S ^{[1
]}

MORSHIDI, MB ^{[1
]}

MANSHARDT, R ^{[1
]}

机构：

[1] UNIV HAWAII,COLL TROP AGR & HUMAN RESOURCES,DEPT HORT,HONOLULU,HI 96822

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 85卷 / 6-7期

关键词：

CARICA-PAPAYA L; POLYMERASE CHAIN REACTION; GENETIC POLYMORPHISM; PHYLOGENETIC RELATIONSHIP;

D O I：

10.1007/BF00225007

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We have applied the recently developed technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among ten cultivars of papaya (Carica papaya L.). Eleven ten-base synthetic oligonucleotides were chosen that gave multiple PCR amplification products using papaya DNA as template. These 11 primers amplified a total of 102 distinct fragments. Cultivars were scored for presence or absence of RAPD fragments and grouped by cluster analysis using simple matching coefficients of similarity. A dendrogram of the ten cultivars was constructed. Of the ten cultivars seven were of the Hawaiian type, and all of these grouped to one branch of the tree. Divisions within the Hawaiian, branch were mostly consistent with the known genetic background of these cultivars. Three non-Hawaiian, cultivars were also analyzed. The minimum similarity detected was 0.7 suggesting that the domesticated papaya germ plasm is quite narrow. Our results show that RAPD technology is a rapid, precise and sensitive technique for genomic analysis.

引用

页码：697 / 701

页数：5

共 16 条

[1]

Chan Y. K., 1985, MARDI Research Bulletin, V13, P1

[2]

CHAN YK, 1987, MALAYS APPL BIOL, V16, P95

[3]

Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]

[4]

Dunn G., 1982, INTRO MATH TAXONOMY

[5] PHYSICAL MAPPING OF TEMPERATURE-SENSITIVE MUTATIONS OF ADENOVIRUSES [J].

GRODZICKER, T ;

WILLIAMS, J ;

SHARP, P ;

SAMBROOK, J .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1974, 39 :439-446

[6]

HAMILTON RA, 1968, 69 HAW AGR EXP STN C

[7]

Jaccard P., 1908, B SOC VAUD SCI N, V44, P223

[8]

LANDER ES, 1989, GENETICS, V121, P185

[9]

NAKASONE HY, 1972, HAWAII AGR EXP STN T, V79

[10] RESOLUTION OF QUANTITATIVE TRAITS INTO MENDELIAN FACTORS BY USING A COMPLETE LINKAGE MAP OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS [J].

PATERSON, AH ;

LANDER, ES ;

HEWITT, JD ;

PETERSON, S ;

LINCOLN, SE ;

TANKSLEY, SD .

NATURE, 1988, 335 (6192) :721-726

← 1 2 →