SODIUM-NITROPRUSSIDE DEGENERATES CULTURED RAT STRIATAL NEURONS

被引：38

作者：

CHEN, J

CHANG, B

WILLIAMS, M

MURAD, F

机构：

[1] Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL

来源：

NEUROREPORT | 1991年 / 2卷 / 03期

关键词：

NITRIC OXIDE; CYCLIC GMP; NEURONAL DEATH;

D O I：

10.1097/00001756-199103000-00002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

INCUBATION of a primary culture of rat striatal neurons with sodium nitroprusside (SNP), a known source of nitric oxide (NO), showed a concentration-dependent increase in cyclic GMP levels with an EC50 value of 13.7-mu-M. Twenty-four hours following incubation with 10-mu-M SNP, striatal neurons underwent degeneration as assessed immunohistochemically and biochemically. In contrast, potassium ferricyanate at concentrations up to 1 mM had no effect on striatal neuron viability. These results indicate that SNP has neurotoxic actions in-vitro, an effect that may involve NO as a second messenger.

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收藏

页码：121 / 123

页数：3

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