CALMODULIN-BINDING PROTEINS OF SACCHAROMYCES-CEREVISIAE

被引：18
作者：
LIU, Y
YAMASHITA, Y
TSUCHIYA, E
MIYAKAWA, T
机构：
[1] Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90863-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The subcellular distribution of calmodulin-binding proteins in the soluble, plasma membrane, and nuclear fractions of Saccharomyces cerevisiae was analyzed with a gel binding assay using 125I-labeled calmodulin. Over 20 binding proteins were detected. The calmodulin-binding protein profiles were markedly different among the fractions. Calmodulin-binding proteins were most abundant in the nuclear fraction, followed by the membrane fraction and the soluble fraction in decreasing order. The amounts of certain calmodulin-binding proteins increased after treatment with α-mating factor. © 1990.
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页码：681 / 686
页数：6
相关论文
共 16 条
[1]   PROTEIN IODINATION WITH SOLID-STATE LACTOPEROXIDASE [J].
DAVID, GS ;
REISFELD, RA .
BIOCHEMISTRY, 1974, 13 (05) :1014-1021
[2]   ISOLATION OF THE YEAST CALMODULIN GENE - CALMODULIN IS AN ESSENTIAL PROTEIN [J].
DAVIS, TN ;
URDEA, MS ;
MASIARZ, FR ;
THORNER, J .
CELL, 1986, 47 (03) :423-431
[3]   CHITIN SYNTHETASE ZYMOGEN IS ATTACHED TO YEAST PLASMA-MEMBRANE [J].
DURAN, A ;
BOWERS, B ;
CABIB, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3952-3955
[4]  
ELDIK LJV, 1983, J BIOL CHEM, V258, P4539
[5]  
GLENNEY JR, 1983, METHOD ENZYMOL, V102, P204
[6]   CA2+-INDUCED HYDROPHOBIC SITE ON CALMODULIN - APPLICATION FOR PURIFICATION OF CALMODULIN BY PHENYL-SEPHAROSE AFFINITY-CHROMATOGRAPHY [J].
GOPALAKRISHNA, R ;
ANDERSON, WB .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 104 (02) :830-836
[7]  
HARRIS PJ, 1981, MITOSIS CYTOKINESIS, P29
[8]   ISOLATION AND CHARACTERIZATION OF THE NUCLEI FROM SACCHAROMYCES-CEREVISIAE [J].
KAMIMURA, K ;
TSUCHIYA, E ;
MIYAKAWA, T ;
FUKI, S ;
HIRATA, A .
CURRENT MICROBIOLOGY, 1981, 6 (03) :175-180
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE IN SACCHAROMYCES-CEREVISIAE [J].
LONDESBOROUGH, J ;
NUUTINEN, M .
FEBS LETTERS, 1987, 219 (01) :249-253
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