INFLUENCE OF STATIONARY-PHASE CHEMISTRY ON SHAPE-RECOGNITION IN LIQUID-CHROMATOGRAPHY

Molecular shape recognition is examined for a series of C-18 columns prepared using a variety of synthetic approaches. Mono-, di-, and trifunctional silanes are used to prepare stationary phases through monomeric and polymeric surface modification procedures, including an approach employing self-assembled monolayer technology. Shape discrimination properties of the columns were investigated with various nonplanar, planar, and linear polycyclic aromatic hydrocarbon solute probes, Chromatographic retention behavior is examined in the context of recently proposed statistical mechanical ''interphase'' retention models.