MOLECULAR TOOLS FOR THE ANALYSIS OF CELL-ADHESION

被引：8

作者：

FAWCETT, J ^{[1
]}

HOLNESS, CLL ^{[1
]}

SIMMONS, DL ^{[1
]}

机构：

[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CAANC RES FUND,CELL ADHES LAB,OXFORD OX3 9DU,ENGLAND

来源：

CELL ADHESION AND COMMUNICATION | 1994年 / 2卷 / 03期

关键词：

CLONING; ICAM-3; CD44; SOLUBLE CAMS;

D O I：

10.3109/15419069409004448

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Advances in cell adhesion research have often been linked to technological advances. The development of monoclonal antibody technology allowed the definition of the molecular components of cell adhesion and pointed to the complexity of the leukocyte cell surface (Knapp & Gilks, 1989); previously only the erythrocyte had been subjected to attempts at detailed analysis of membrane associated molecules (Steck, 1974). It was also possible to use selection strategies to produce antibodies that could perturb functional properties of the antigens they recognise. The interaction between the T cell surface molecule CD2 and its natural ligand LFA-3 was defined by monoclonal antibodies which blocked adhesion and the same antibodies have allowed large scale purification of these proteins for detailed in vitro studies (Dustin & Springer, 1991). © 1994 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：275 / 285

页数：11

共 21 条

[1] CD44 IS THE PRINCIPAL CELL-SURFACE RECEPTOR FOR HYALURONATE [J].

ARUFFO, A ;

STAMENKOVIC, I ;

MELNICK, M ;

UNDERHILL, CB ;

SEED, B .

CELL, 1990, 61 (07) :1303-1313

[2] ROLE OF LYMPHOCYTE ADHESION RECEPTORS IN TRANSIENT INTERACTIONS AND CELL LOCOMOTION [J].

DUSTIN, ML ;

SPRINGER, TA .

ANNUAL REVIEW OF IMMUNOLOGY, 1991, 9 :27-66

[3] MOLECULAR-CLONING OF ICAM-3, A 3RD LIGAND FOR LFA-1, CONSTITUTIVELY EXPRESSED ON RESTING LEUKOCYTES [J].

FAWCETT, J ;

HOLNESS, CLL ;

NEEDHAM, LA ;

TURLEY, H ;

GATTER, KC ;

MASON, DY ;

SIMMONS, DL .

NATURE, 1992, 360 (6403) :481-484

[4]

FOUGEROLLES AR, 1991, J EXP MED, V174, P253

[5]

FOUGEROLLES AR, 1992, J EXP MED, V175, P185

[6] CD44 AND CANCER SCREENING [J].

FOX, SB ;

GATTER, KC ;

JACKSON, DG ;

SCREATON, GR ;

BELL, MV ;

BELL, JI ;

HARRIS, AL ;

SIMMONS, D ;

FAWCETT, J .

LANCET, 1993, 342 (8870) :548-549

[7] A NEW VARIANT OF GLYCOPROTEIN CD44 CONFERS METASTATIC POTENTIAL TO RAT CARCINOMA-CELLS [J].

GUNTHERT, U ;

HOFMANN, M ;

RUDY, W ;

REBER, S ;

ZOLLER, M ;

HAUSSMANN, I ;

MATZKU, S ;

WENZEL, A ;

PONTA, H ;

HERRLICH, P .

CELL, 1991, 65 (01) :13-24

[8]

HERNANDEZCASELL.T, IN PRESS J IMMUNOLOG

[9]

HOFMANN M, 1991, CANCER RES, V51, P5292

[10]

KNAPP W, 1989, LEUCOCYTE TYPING, V4

← 1 2 3 →