HYDROGEN-PEROXIDE ELICITS ACTIVATION OF BOVINE PULMONARY ARTERIAL SOLUBLE GUANYLATE-CYCLASE BY A MECHANISM ASSOCIATED WITH ITS METABOLISM BY CATALASE

被引：62

作者：

WOLIN, MS

BURKE, TM

机构：

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1987年 / 143卷 / 01期

关键词：

D O I：

10.1016/0006-291X(87)90623-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Guanylate cyclase activity in the soluble extract of bovine pulmonary arteries is activated by hydrogen peroxide generated by glucose oxidase only in the presence of catalase. This mechanism of guanylate cyclase activation is not blocked by scavengers for superoxide anion or hydroxyl radical, but is selectively inhibited by methylene blue, inactivation of catalase and ethanol. The time dependency of increases in guanylate cyclase activity in the presence of peroxides that are substrates for catalase are associated with the spectral detection of compound I, a species of catalase formed during the metabolism of peroxide. Thus, activation of soluble guanylate cyclase appears to be elicited by compound I of catalase or by a mediator generated by this species.

引用

页码：20 / 25

页数：6

共 17 条

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[2]

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[3]

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