A SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HUMAN INTERLEUKIN-5

被引：33
|
作者：
FUKUDA, Y [1 ]
HASHINO, J [1 ]
HARUYAMA, M [1 ]
TSURUOKA, N [1 ]
NAKAZATO, H [1 ]
NAKANISHI, T [1 ]
机构：
[1] SUNTORY INST BIOMED RES,1-1-1 WAKAYAMADAI,SHIMAMOTO CHO,OSAKA 618,JAPAN
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 143卷 / 01期
关键词：
INTERLEUKIN-5; ELISA; SANDWICH; MONOCLONAL ANTIBODY;
D O I：
10.1016/0022-1759(91)90276-L
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for human interleukin-5 (hIL-5) using a combination of monoclonal anti-recombinant(r)-hIL-5 antibody and rabbit anti-r-hIL-5 IgG. Detection limit of this assay was estimated to be 7.8 pg/ml, which was about 10,000 times more sensitive than that of the bioassay using BCL1 cells of murine origin. This ELISA was specific for hIL-5, showing no crossreactivity to recombinant human GM-CSF, IL-4, TNF-alpha, IFN-gamma and mouse IL-5 (mIL-5). The presence of 10% fetal calf serum did not interfere with the measurement of r-hIL-5. Coefficients of variation in intra-assay and interassay were 1.1-4.6% and 2.3-11.3%, respectively. These results indicate that this assay system can be quite useful in quantifying hIL-5 in various biological fluids.
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页码：89 / 94
页数：6
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