Sodium fluoride (NaF) induced changes in growth and DNA profile of Vigna radiata

被引：9

作者：

Agarwal, Swati ^{[1
]}

Khan, Suphiya ^{[1
]}

机构：

[1] Banasthali Univ, Dept Biosci & Biotechnol, PO Banasthali Vidyapith, Vanasthali 304022, Rajasthan, India

来源：

PLANT SCIENCE TODAY | 2016年 / 3卷 / 01期

关键词：

Fluoride; GTS; RAPD; Vigna radiata;

D O I：

10.14719/pst.2016.3.1.137

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Fluoride (F) is a natural, ubiquitous, non-biodegradable and hazardous pollutant. To investigate its effect, Vigna radiata seeds were treated with NaF (0, 25, 50, 75, 100 mg NaF kg-1) and its effect was studied on germination, growth and RAPD profile. The variation in DNA profile in response to NaF treatment was detected by RAPD-PCR technique. The result of RAPD was observed in terms of GTS% (Genomic Template Stability). Results showed that there was gradual decrease in GTS% i.e. increase in DNA damage from 25 to 75 mg NaF kg-1 concentration. These data demonstrate that RAPD is a reliable tool and permits greater insights into the genetic alteration of V. radiata.

引用

页码：1 / 5

页数：5

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