STIMULATION OF PRENYLATED FLAVANONE PRODUCTION BY MANNANS AND ACIDIC POLYSACCHARIDES IN CALLUS-CULTURE OF SOPHORA-FLAVESCENS

被引：20
作者：
YAMAMOTO, H
ICHIMURA, M
INOUE, K
机构：
[1] Department of Pharmacognosy, Gifu Pharmaceutical University, Gifu, 502
来源：
PHYTOCHEMISTRY | 1995年 / 40卷 / 01期
关键词：
SOPHORA FLAVESCENS; LEGUMINOSAE; CALLUS CULTURE; PRENYLATED FLAVANONE; SOPHORA-FLAVANONE G; LEHMANNIN; YEAST EXTRACT; MANNAN; PECTIN; PECTINIC ACID; ELICITOR;
D O I：
10.1016/0031-9422(95)00178-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of polysaccharides on the production of prenylated flavanones sophoraflavanone G and lehmannin in Sophora flavescens callus culture was investigated. The production of these flavanones was stimulated up to 5 times by the addition of 2 mg/ml yeast extract. The active component was partially purified as a mannan (c 92% of mannose and c 5% glucose) by DEAE-cellulose and Concanavalin A-Sepharose column chromatographies. Acidic polysaccharides also stimulated the production of prenylated flavanones. The effect of pectin was higher than that of pectinic acid from the same source. Other polysaccharides such as cellulose, beta-1,3-glucan and chitosan did not influence the productivity.
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页码：77 / 81
页数：5
相关论文
共 16 条
[1]   OLIGOSACCHARINS - OLIGOSACCHARIDES THAT REGULATE GROWTH, DEVELOPMENT AND DEFENSE RESPONSES IN PLANTS [J].
DARVILL, A ;
AUGUR, C ;
BERGMANN, C ;
CARLSON, RW ;
CHEONG, JJ ;
EBERHARD, S ;
HAHN, MG ;
LO, VM ;
MARFA, V ;
MEYER, B ;
MOHNEN, D ;
ONEILL, MA ;
SPIRO, MD ;
VANHALBEEK, H ;
YORK, WS ;
ALBERSHEIM, P .
GLYCOBIOLOGY, 1992, 2 (03) :181-198
[2]  
FARMER EE, 1991, J BIOL CHEM, V266, P3140
[3]  
Fujita Y, 1981, Plant Cell Rep, V1, P61, DOI 10.1007/BF00269273
[4]   BIOLOGICAL INTERACTIONS BETWEEN POLYSACCHARIDES AND DIVALENT CATIONS - EGG-BOX MODEL [J].
GRANT, GT ;
MORRIS, ER ;
REES, DA ;
SMITH, PJC ;
THOM, D .
FEBS LETTERS, 1973, 32 (01) :195-198
[5]  
HARN MG, 1978, PLANT PHYSIOL, V62, P107
[6]   PECTIC ENZYMES [J].
KERTESZ, ZI .
METHODS IN ENZYMOLOGY, 1955, 1 :158-166
[7]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[8]  
SCHUMACHER HM, 1987, PLANT CELL REP, V6, P410, DOI 10.1007/BF00272770
[9]   DETERMINATION OF ALDOSES AND URONIC-ACID CONTENT OF VEGETABLE FIBER [J].
SELVENDRAN, RR ;
MARCH, JF ;
RING, SG .
ANALYTICAL BIOCHEMISTRY, 1979, 96 (02) :282-292
[10]  
SONG KS, 1994, IN PRESS PHYTOCHEMIS
12