THE BLASTICIDIN-S RESISTANCE GENE (BSR) SELECTABLE IN A SINGLE COPY STATE IN THE BACILLUS-SUBTILIS CHROMOSOME

被引：48

作者：

ITAYA, M

YAMAGUCHI, I

KOBAYASHI, K

ENDO, T

TANAKA, T

机构：

[1] INST PHYS & CHEM RES,WAKO,SAITAMA 35101,JAPAN

[2] KYORITSU COLL PHARMACEUT SCI,MINATO KU,TOKYO 105,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 107卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123128

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported. © 1990 COPYRIGHT, 1990 BY The Journal of Biochemistry.

引用

页码：799 / 801

页数：3

共 12 条

[1]

Dubnau DA, 1982, MOL BIOL BACILLI, VI, P148

[2] INACTIVATION OF BLASTICIDIN-S BY BACILLUS-CEREUS .2. ISOLATION AND CHARACTERIZATION OF A PLASMID, PBSR8, FROM BACILLUS-CEREUS [J].