A SINGLE-STEP DNA EXTRACTION PROCEDURE FOR THE DETECTION OF SERUM HEPATITIS-B VIRUS SEQUENCES BY THE POLYMERASE CHAIN-REACTION

被引：17
作者：
MANZIN, A
SALVONI, G
BAGNARELLI, P
MENZO, S
CARLONI, G
CLEMENTI, M
机构：
[1] UNIV ANCONA,REG HOSP TORRETTE,SCH MED,INST MICROBIOL,VIROL UNIT,I-60100 ANCONA,ITALY
[2] CNR,INST EXPTL MED,ROME,ITALY
[3] UNIV TRIESTE,INST MICROBIOL,I-34100 TRIESTE,ITALY
来源：
JOURNAL OF VIROLOGICAL METHODS | 1991年 / 32卷 / 2-3期
关键词：
DNA EXTRACTION; HEPATITIS-B VIRUS; POLYMERASE CHAIN REACTION;
D O I：
10.1016/0166-0934(91)90055-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid single-step procedure for the isolation of low molecular weight DNA using guanidinium thiocyanate and phenol as protein denaturants is described and applied for the detection of specific hepatitis B virus (HBV) DNA sequences from serum samples by the polymerase chain reaction (PCR). The novel technique is efficient and, when compared to the standard proteinase K/phenol/chloroform method has the advantage of being faster and easily adaptable to the routine processing of a high number of clinical samples by PCR and spot hybridization techniques.
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页码：245 / 253
页数：9
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