INHIBITION OF BETA-GALACTOSIDASE BIOSYNTHESIS IN ESCHERICHIA-COLI - EFFECT OF ALTERATIONS OF THE OUTER-MEMBRANE PERMEABILITY ON SENSITIVITY TO ENVIRONMENTAL TOXICANTS

被引：5
作者：
DUTTON, RJ
BITTON, G
KOOPMAN, B
AGAMI, O
机构：
[1] Laboratory of Environmental Microbiology, Department of Environmental Engineering Sciences, University of Florida, Gainesville, Florida
来源：
TOXICITY ASSESSMENT | 1990年 / 5卷 / 03期
关键词：
D O I：
10.1002/tox.2540050305
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
The use of bacteria as test organisms in rapid ecotoxicity assays is under investigation in several laboratories worldwide. However, little attention has been given to the question of permeability of the outer membrane of gram‐negative bacteria to toxicants of environmental interest. We have investigated the effect of physical, chemical, and genetic alterations on the sensitivity of Escherichia coli to environmental toxicants, as measured via inhibition of β‐galactosidase biosynthesis. Polymyxin treatment (2 mg/L) was the most promising treatment tested, significantly increasing the sensitivity of wild‐type E. coli to pentachlorophenol and sodium dodecyl sulfate. A mutant strain of E. coli (EW1b), with an outer membrane protein alteration (tol C gene), was found to be the most sensitive to hydrophobic compounds and to detergent. EW1b, further sensitized via polymyxin treatment, appears to be a sensitive microorganism for toxicant assay. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company
引用
收藏
页码：253 / 264
页数：12
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