EFFECT OF CRYOGENIC STORAGE ON HUMAN ERYTHROCYTE-MEMBRANE PROTEINS AS DETERMINED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：6
作者：
SKRABUT, EM
CROWLEY, JP
CATSIMPOOLAS, N
VALERI, CR
机构：
[1] USN, BLOOD RES LAB, CHELSEA, MA 02150 USA
[2] HARVARD UNIV, PETER BENT BRIGHAM HOSP, SCH MED, DEPT MED, BOSTON, MA 02115 USA
[3] BOSTON UNIV, MED CTR, DEPT HEMATOL, BOSTON, MA 02118 USA
[4] MIT, DEPT NUTR & FOOD SCI, BIOPHYS LAB, CAMBRIDGE, MA 02139 USA
来源：
CRYOBIOLOGY | 1976年 / 13卷 / 04期
关键词：
D O I：
10.1016/0011-2240(76)90095-X
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A study was conducted to determine the effects of freezing on the major membrane proteins of isolated human erythrocyte membranes. Membranes in low or normal ionic strength medium were frozen at slow or fast freezing rates. The membrane protein composition and elution of proteins from the membranes were studied utilizing polyacrylamide-gel electrophoresis in a sodium dodecyl sulfate or an acetic acid-urea-phenol solvent system. Neither a change in the composition of the membrane proteins nor any elution of membrane protein during freezing and thawing was observed. Any human erythrocyte membrane damage during freezing and thawing was not related to a change in major membrane protein composition. Human red cell membranes were stable at -80 or -196.degree. C in the absence of a cryoprotective agent.
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收藏
页码：395 / 403
页数：9
相关论文
共 33 条
[1]   NEW PROCEDURES FOR ISOLATING POLYPEPTIDES + PROTEINS FROM TISSUES - METABOLIC INCORPORATION OF L-(14C)VALINE INTO FRACTIONS OF INTERMEDIATE MOLECULAR WEIGHT IN BROAD-BEAN ) VICIA FABA L ) LEAVES [J].
BAGDASARIAN, M ;
SYNGE, RLM ;
MATHESON, NA ;
YOUNGSON, MA .
BIOCHEMICAL JOURNAL, 1964, 91 (01) :91-&
[2]   PROTEIN SUBUNITS IN DORMANT AND GERMINATING SOYBEAN SEEDS [J].
CATSIMPOOLAS, N ;
EKENSTAM, C ;
ROGERS, DA ;
MEYER, EW .
BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 168 (01) :122-+
[3]   DITHIOTHREITOL NEW PROTECTIVE REAGENT FOR SH GROUPS [J].
CLELAND, WW .
BIOCHEMISTRY, 1964, 3 (04) :480-&
[4]   CHANGES IN PROTEINS OF MILK DESTABILIZED DURING FROZEN STORAGE [J].
DESAI, ID ;
NICKERSON, TA .
NATURE, 1964, 202 (492) :183-&
[5]   ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].
FAIRBANKS, G ;
STECK, TL ;
WALLACH, DFH .
BIOCHEMISTRY, 1971, 10 (13) :2606-+
[6]  
FAIRBANKS G, 1969, THESIS MASSACHUSETTS
[7]   MECHANISM OF CELL DAMAGE DURING FREEZING AND THAWING AND ITS PREVENTION [J].
FARRANT, J .
NATURE, 1965, 205 (4978) :1284-&
[8]  
HARRIS GC, 1970, CRYOBIOLOGY, V7, P209, DOI 10.1016/0011-2240(70)90023-4
[9]  
HECHTER O, 1965, FED PROC, V24, pS91
[10]   ACTIVE TRANSPORT OF SODIUM BY GHOSTS OF HUMAN RED BLOOD CELLS [J].
HOFFMAN, JF .
JOURNAL OF GENERAL PHYSIOLOGY, 1962, 45 (05) :837-&
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