CHITINASE AND CHITIN SYNTHASE .1. COUNTERBALANCING ACTIVITIES IN CELL-SEPARATION OF SACCHAROMYCES-CEREVISIAE

被引:120
作者
CABIB, E
SILVERMAN, SJ
SHAW, JA
机构
[1] Lab. of Biochem. and Metabol., National Inst. of Diabetes, National Institutes of Health, Bethesda
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷
关键词
D O I
10.1099/00221287-138-1-97
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Previous results [E. Cabib, A. Sburlati, B. Bowers & S. J. Silverman (1989) Journal of Cell Biology 108, 1665-1672] strongly suggested that the lysis observed in daughter cells of Saccharomyces cerevisiae defective in chitin synthase 1 (Chs1) was caused by a chitinase that partially degrades the chitin septum in the process of cell separation. Consequently, it was proposed that in wild-type cells, Chs1 acts as a repair enzyme by replenishing chitin during cytokinesis. The chitinase requirement for lysis has been confirmed in two different ways: (a) demethylallosamidin, a more powerful chitinase inhibitor than the previously used allosamidin, is also a much better protector against lysis and (b) disruption of the chitinase gene in chs1 cells eliminates lysis. Reintroduction of a normal chitinase gene, by transformation of those cells with a suitable plasmid, restores lysis. The percentage of lysed cells in strains lacking Chs1 was not increased by elevating the chitinase level with high-copy-number plasmids carrying the hydrolase gene. Furthermore, the degree of lysis varied in different chs1 strains; lysis was abolished in chs1 mutants containing the scs1 suppressor. These results indicate that, in addition to chitinase, lysis requires other gene products that may become limiting.
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页码:97 / 102
页数:6
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