A BACTERIOPHAGE-LAMBDA VECTOR FOR CLONING WITH BAMHI AND SAU3A

被引：72

作者：

MIZUSAWA, S ^{[1
]}

WARD, DF ^{[1
]}

机构：

[1] NCI, MOLEC BIOL LAB, BETHESDA, MD 20205 USA

来源：

GENE | 1982年 / 20卷 / 03期

关键词：

D O I：

10.1016/0378-1119(82)90200-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A phage .lambda. cloning vector was constructed which contains a single site for the restriction endonuclease BamHI. Since Sau3A and BglII produce the same cohesive ends as BamHI, this vector can also be used to clone DNA fragments generated with either of these enzymes. This vector was used to construct an Escherichia coli library using partial digestion with Sau3A. This vector will be most useful for applications requiring genetic analysis of cloned E. coli genes.

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页码：317 / 322

页数：6

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