A STUDY OF TRIPLEX FORMATION OF 5'-D-T-(C-T-)(2)C-(T-)(4)C-(T-C-)(2)T WITH 5'-D-A-(G-A-)(2)G - A HAIRPIN TRIPLEX WITH 3 T-CENTER-DOT-A-CENTER-DOT-T AND 3 C+CENTER-DOT-G-CENTER-DOT-C BASES TRIADS

被引：10

作者：

TSAY, LM

LIN, SB

TSAY, HT

CHEN, HH

KAN, LS

机构：

[1] NATL TAIWAN UNIV,GRAD INST MED TECHNOL,TAIPEI,TAIWAN

[2] CULTURE UNIV,INST APPL CHEM,TAIPEI,TAIWAN

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1995年 / 12卷 / 06期

关键词：

D O I：

10.1080/07391102.1995.10508809

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The UV mixing titration, gel electrophoresis, and CD measurements indicate that oligomers with a basic sequence of 5'-d-T-(C-T-)(2)C-(T-)(4)C-(T-C-)(2)T form a hairpin type triplex with the target 5'-d-A-(G-A-)(2)G. The stability, measured UV melting temperatures, were studied in aqueous solution as functions of C-m (5-methylcytidine) replacement of C, pH (4 to 7), and salt concentration (up to 1 M). The order of stability is 5'-d-A-(G-A-)(2)G + 5'-d-T-(C-T-)(2)C-(T-)(4)C-(T-C-)(2)T < 5'-d-A-(G-A-)(2)G + 5'-d-T-(C-m-T-)(2)C-m-(T-)(4)C-(T-C-)(2)T approximate to 5'-d-A-(G-A-)(2)G + 5'-d-T-(C-T-)(2)C-(T-)(4)C-m-(T-C-m-)(2)T < 5'-d-A-(G-A-)(2)G + 5'-d-T-(C-m-T-)(2)C-m-(T-)(4)C-m-(T-C-m-)(2)T at pH 4. These results indicate that (a) a stable tripler is formed with three T . A . T and three C+. G . C base triads and (b) C-m is more effective than C to stablize the tripler formation in acidic condition. Thus, this provides a simple system for further studies of tripler.

引用

页码：1235 / 1245

页数：11

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