EFFECTS OF PYRIDOXAL 5'-PHOSPHATE ON THE REFOLDING OF ASPARTATE-AMINOTRANSFERASE

被引：1

作者：

YOSHIMURA, T ^{[1
]}

WAKAYAMA, M ^{[1
]}

KIM, DW ^{[1
]}

ESAKI, N ^{[1
]}

SODA, K ^{[1
]}

机构：

[1] KYOTO UNIV, INST CHEM RES, UJI, KYOTO 611, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 02期

关键词：

D O I：

10.1271/bbb.58.363

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of pyridoxal 5'-phosphate on the refolding of aspartate aminotransferase were studied. The refolding of the cytoplasmic isozyme of pig heart aspartate aminotransferase denatured with 6M guanidine hydrochloride depends critically on the presence of pyridoxal 5'-phosphate. Pyridoxal 5'-phosphate affects a refolding intermediate at guanidine hydrochloride concentrations between 3.5 and 2.0 M to bring out the correct folding. In contrast, thermostable aspartate aminotransferase of Bacillus sp. YM-2 can be refolded independently of pyridoxal 5'-phosphate.

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页码：363 / 365

页数：3

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