HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BETAMETHASONE AND DEXAMETHASONE

被引：15
作者：
LIU, KR [1 ]
CHEN, SH [1 ]
WU, SM [1 ]
KOU, HS [1 ]
WU, HL [1 ]
机构：
[1] KAOHSIUNG MED COLL,GRAD INST PHARMACEUT SCI,KAOHSIUNG 807,TAIWAN
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 676卷 / 02期
关键词：
D O I：
10.1016/0021-9673(94)80447-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method was developed for the determination of glucocorticoid epimers of betamethasone (BTM) and dexamethasone (DXM) by high-performance liquid chromatography with UV detection, using silica as the stationary phase and dichloromethane-ethanol (34:1, v/v) as the mobile phase. The linear range of the method for the determination of BTM and DXM in 1.0 ml of sample solution was over 5-50 nmol. The detection limits (signal-to-noise ratio = 5) of BTM and DXM with an injection volume of 25 mu l were 80 and 60 pmol, respectively. The method was satisfactorily applied to the individual determination of BTM and DXM in commercial tablets.
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页码：455 / 460
页数：6
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