HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BETAMETHASONE AND DEXAMETHASONE

被引：15

作者：

LIU, KR ^{[1
]}

CHEN, SH ^{[1
]}

WU, SM ^{[1
]}

KOU, HS ^{[1
]}

WU, HL ^{[1
]}

机构：

[1] KAOHSIUNG MED COLL,GRAD INST PHARMACEUT SCI,KAOHSIUNG 807,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 676卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80447-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method was developed for the determination of glucocorticoid epimers of betamethasone (BTM) and dexamethasone (DXM) by high-performance liquid chromatography with UV detection, using silica as the stationary phase and dichloromethane-ethanol (34:1, v/v) as the mobile phase. The linear range of the method for the determination of BTM and DXM in 1.0 ml of sample solution was over 5-50 nmol. The detection limits (signal-to-noise ratio = 5) of BTM and DXM with an injection volume of 25 mu l were 80 and 60 pmol, respectively. The method was satisfactorily applied to the individual determination of BTM and DXM in commercial tablets.

引用

页码：455 / 460

页数：6

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