ONE-STEP IMMUNOAFFINITY PURIFICATION OF COMPLEX-I SUBUNITS FROM BEEF-HEART MITOCHONDRIA

被引：3
作者：
HAINES, AMR
COOPER, JM
MORGANHUGHES, JA
CLARK, JB
SCHAPIRA, AHV
机构：
[1] INST NEUROL, DEPT CLIN NEUROL, LONDON WC1N 3BG, ENGLAND
[2] INST NEUROL, DEPT NEUROCHEM, LONDON WC1N 3BG, ENGLAND
来源：
PROTEIN EXPRESSION AND PURIFICATION | 1992年 / 3卷 / 03期
关键词：
D O I：
10.1016/1046-5928(92)90018-R
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Polypeptides of beef heart mitochondrial complex I were isolated from 15 mg of solubilized beef heart mitochondria using antibodies immobilized on an agarose chromatography column. The preparation was examined by SDS electrophoresis and Western blotting using affinity-purified antibodies to complex I and compared to beef heart complex I purified according to the conventional method of Hatefi and Rieske. There was a high degree of homology between the two preparations as judged by SDS-polyacrylamide electrophoresis and by immunoblotting with seven affinity-purified antibodies to various complex I subunits. This method could be applied to the preparation of complex I subunits from small samples such as human muscle biopsy specimens. © 1992.
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页码：223 / 227
页数：5
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