Development of a DNA-based Macroarray for the Detection and Identification of Fusarium oxysporum f. sp. vasinfectum in Cotton Tissue

被引：0

作者：

Gilbert, C. ^{[1
]}

Zhang, N. ^{[2
]}

Hutmacher, R. ^{[3
]}

Davis, R. ^{[1
]}

Smart, C. ^{[2
]}

机构：

[1] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

[2] Cornell Univ, Dept Plant Pathol, Geneva, NY 14456 USA

[3] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

JOURNAL OF COTTON SCIENCE | 2008年 / 12卷 / 02期

基金：

美国农业部;

关键词：

D O I：

暂无

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A DNA-based macroarray was developed to quickly and accurately identify all known races and the two Australian biotypes of Fusarium oxysporum f. sp. vasinfectum, the causal agent of Fusarium wilt of cotton. The macroarray utilized oligonucleotide probes designed from sequences of the elongation factor gene unique to Races 1, 3, 4, 8 or the Australian biotypes. Starting with diseased tissue, the entire detection process from DNA extraction to race identification could be completed within 8 hours. The assay reproducibly identified all F. oxysporum f. sp. vasinfectum races and biotypes from cultures, and greenhouse-or field-grown infected cotton plants.

引用

页码：165 / 170

页数：6

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