RAPID TRANSLOCATION OF CYTOSOLIC CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II INTO POSTSYNAPTIC DENSITY AFTER DECAPITATION

被引:106
作者
SUZUKI, T [1 ]
OKUMURANOJI, K [1 ]
TANAKA, R [1 ]
TADA, T [1 ]
机构
[1] NAGOYA CITY UNIV,SCH MED,DEPT PATHOL,NAGOYA,AICHI,JAPAN
关键词
POSTSYNAPTIC DENSITY PROTEIN; RAT FOREBRAIN; CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE II; PARTICULATE; CYTOSOL;
D O I
10.1046/j.1471-4159.1994.63041529.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The postsynaptic density (PSD) fraction prepared from rat forebrains frozen with liquid nitrogen immediately after dissection (within 30 s after decapitation) contained major postsynaptic density protein (mPSDp), ct subunit of Ca2+/calmodulin-dependent protein kinase II (CaMKII) at a level of merely 2.7% of the total protein. The content of the protein in the fraction was increased to similar to 10% by placing the forebrains on ice for a few minutes. Accumulation, but to a lesser extent, of the protein after placement was also observed in the particulate, synaptosome, and synaptic plasma membrane fractions with its concomitant decrease in the cytosolic fraction. The distribution change may be translocation of the protein, because the amounts of the losses of the protein in the cytosolic fraction were balanced by the gains in the particulate fractions. By translocation, CaMKII became Triton X-100 insoluble and partially inactivated. The amount of CaMKII transferred from the cytosol to particulate fractions at 0 degrees C was about the same as that contained in the conventional PSD fraction. Furthermore, the thickness of the PSD was increased by the treatment of the forebrains at 37 degrees C, by which the content of CaMKII alpha in the PSD fraction was increased to twofold. These results suggest that most of the CaMKII alpha subunit associated with the PSD fraction (mPSDp) is translocated from cytosol after decapitation. We also showed similar translocation of CaMKII beta/beta'.
引用
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页码:1529 / 1537
页数:9
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