ROLE OF ELEVATED MONOCYTE TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) PRODUCTION IN POSTTRAUMA IMMUNOSUPPRESSION

被引：101

作者：

MILLERGRAZIANO, CL ^{[1
]}

SZABO, G ^{[1
]}

GRIFFEY, K ^{[1
]}

MEHTA, B ^{[1
]}

KODYS, K ^{[1
]}

CATALANO, D ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,MED CTR,DEPT MOLEC GENET & MICROBIOL,WORCESTER,MA 01655

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 1991年 / 11卷 / 02期

关键词：

MONOCYTES; TRANSFORMING GROWTH FACTOR BETA; TRAUMA PATIENTS; PROSTAGLANDIN-E2;

D O I：

10.1007/BF00917745

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We previously reported that increased production of prostaglandin E2 by monocytes is a pivotal mechanism in posttrauma immunopathology. Here we characterize monocyte levels of transforming growth factor-beta and examine the effects of elevated transforming growth factor-beta on prostaglandin E2 release by patients' monocytes. Trauma patients' and normals' monocyte supernates (+/- stimulation with muramyl dipeptide) were acid treated and assayed for transforming growth factor-beta using the mink lung-cell bioassay. Alternatively, human transforming growth factor-beta was added to patients' and normals' monocytes and prostaglandin E2 production assayed. Significantly elevated transforming growth factor-beta levels (median = 181.7 pmol/10(6) monocytes) were detected in immunosuppressed patients' monocytes but not immunocompetent trauma patients' (median = 32.0 pM) or normals' (median = 20.4 pM) monocytes. Adding transforming growth factor-beta to monocytes resulted in a significant elevation of prostaglandin E2 levels. Elevated monocyte transforming growth factor-beta levels in trauma patients could be both suppressing T-lymphocyte functions and maintaining elevated monocyte prostaglandin E2 synthesis.

引用

页码：95 / 102

页数：8

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