ONE-STEP SEPARATION OF HUMAN FETAL LYMPHOCYTES FROM NUCLEATED RED-BLOOD-CELLS

被引：16

作者：

BHAT, NM ^{[1
]}

BIEBER, MM ^{[1
]}

TENG, NNH ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT OBSTET & GYNECOL,STANFORD,CA 94305

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期

关键词：

Density gradient centrifugation; Fetal lymphocyte; Nucleated red blood cell;

D O I：

10.1016/0022-1759(90)90245-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a one step discontinuous density gradient centrifugation procedure to obtain a 20-fold enrichment of human fetal lymphoid cells, reducing contamination from nucleated red blood cells to less than 1%. © 1990.

引用

页码：147 / 149

页数：3

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[2]

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[3] SINGLE-STEP SEPARATION OF RED BLOOD-CELLS, GRANULOCYTES AND MONONUCLEAR LEUKOCYTES ON DISCONTINUOUS DENSITY GRADIENTS OF FICOLL-HYPAQUE [J].

ENGLISH, D ;

ANDERSEN, BR .

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[4]

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[5]

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[6]

Trolle D., 1947, ACTA OBSTET ET GYNECOL SCAND, V27, P327

[7]

VACCA LL, 1985, LABORATORY MANUAL HI, P109

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