A GENERAL-METHOD OF ANALYSIS OF LIGAND MACROMOLECULE EQUILIBRIA USING A SPECTROSCOPIC SIGNAL FROM THE LIGAND TO MONITOR BINDING - APPLICATION TO ESCHERICHIA-COLI SINGLE-STRAND BINDING-PROTEIN NUCLEIC-ACID INTERACTIONS

被引:113
作者
BUJALOWSKI, W
LOHMAN, TM
机构
[1] TEXAS A&M UNIV,DEPT BIOCHEM & BIOPHYS,COLLEGE STN,TX 77843
[2] TEXAS A&M UNIV,DEPT CHEM,COLLEGE STN,TX 77843
关键词
D O I
10.1021/bi00385a023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:3099 / 3106
页数:8
相关论文
共 33 条
[1]   FLUORESCENCE STUDIES OF THE COMPLEX-FORMATION BETWEEN THE GENE-5 PROTEIN OF BACTERIOPHAGE-M13 AND POLYNUCLEOTIDES [J].
ALMA, NCM ;
HARMSEN, BJM ;
DEJONG, EAM ;
VANDERVEN, J ;
HILBERS, CW .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 163 (01) :47-62
[2]   LAMBDA-PHAGE CRO-REPRESSOR - NON-SPECIFIC DNA-BINDING [J].
BOSCHELLI, F .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 162 (02) :267-282
[3]   ESCHERICHIA-COLI SINGLE-STRAND BINDING-PROTEIN FORMS MULTIPLE, DISTINCT COMPLEXES WITH SINGLE-STRANDED-DNA [J].
BUJALOWSKI, W ;
LOHMAN, TM .
BIOCHEMISTRY, 1986, 25 (24) :7799-7802
[4]  
BUJALOWSKI W, 1987, IN PRESS J MOL BIOL
[5]   ESCHERICHIA-COLI SINGLE-STRAND BINDING-PROTEIN ORGANIZES SINGLE-STRANDED-DNA IN NUCLEOSOME-LIKE UNITS [J].
CHRYSOGELOS, S ;
GRIFFITH, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (19) :5803-5807
[6]   MEASUREMENT OF BINDING CONSTANTS FOR PROTEIN-DNA INTERACTIONS BY DNA-CELLULOSE CHROMATOGRAPHY [J].
DEHASETH, PL ;
GROSS, CA ;
BURGESS, RR ;
RECORD, MT .
BIOCHEMISTRY, 1977, 16 (22) :4777-4783
[7]   MEASUREMENT OF MACROMOLECULAR EQUILIBRIUM BINDING CONSTANTS BY A SUCROSE GRADIENT BAND SEDIMENTATION METHOD - APPLICATION TO PROTEIN-NUCLEIC ACID INTERACTIONS [J].
DRAPER, DE ;
VONHIPPEL, PH .
BIOCHEMISTRY, 1979, 18 (05) :753-760
[8]   NUCLEIC-ACID BINDING PROPERTIES OF ESCHERICHIA-COLI RIBOSOMAL PROTEIN-S1 .1. STRUCTURE AND INTERACTIONS OF BINDING SITE-I [J].
DRAPER, DE ;
VONHIPPEL, PH .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 122 (03) :321-338
[9]   STUDIES OF TEMPERATURE-DEPENDENT CONFORMATION AND PHASE SEPARATION OF POLYRIBOADENYLIC ACID SOLUTIONS AT NEUTRAL PH [J].
EISENBERG, H ;
FELSENFELD, G .
JOURNAL OF MOLECULAR BIOLOGY, 1967, 30 (01) :17-+
[10]   EQUILIBRIA AND KINETICS OF LAC REPRESSOR-OPERATOR INTERACTIONS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
FRIED, M ;
CROTHERS, DM .
NUCLEIC ACIDS RESEARCH, 1981, 9 (23) :6505-6525