LIPIDS TRIGGER CHANGES IN THE ELASTICITY OF THE CYTOSKELETON IN PLANT-CELLS - A CELL OPTICAL DISPLACEMENT ASSAY FOR LIVE CELL MEASUREMENTS

被引:38
作者
GRABSKI, S [1 ]
XIE, XG [1 ]
HOLLAND, JF [1 ]
SCHINDLER, M [1 ]
机构
[1] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824
关键词
D O I
10.1083/jcb.126.3.713
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
An assay has been developed to quantitatively measure the tension and elasticity of the cytoskeleton in living plant cells. The cell optical displacement assay (CODA) uses a focused laser beam to optically trap and displace transvacuolar and cortical strands through a defined distance within the cell. Results from these experiments provide evidence for the classification of at least two theologically distinct cytoskeletal assemblies, cortical and transvacuolar, that differ in their tension and response to both signaling molecules and reagents that perturb the cytoskeleton. It is further demonstrated that the tension of the transvacuolar strands can be significantly decreased by the addition of either linoleic acid, 1,2 dioctanoyl-sn-glycerol, or 1,3 dioctanoylglycerol. These decreases in tension could also be induced by lowering the cytoplasmic pH. In contrast, addition of Ca2+, Mg2+, or the ionophore A23187 to the cells caused a considerable increase in the tension of the transvacuolar strands. The data provides evidence that: (a) linoleic acid may be a signaling molecule in plant cells; (b) diacylglycerol functions as a signaling molecule through a protein kinase C-independent pathway mediated by PLA(2); and (c) Ca2+ and pH have regulatory roles for controlling cytoskeleton tension and organization.
引用
收藏
页码:713 / 726
页数:14
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