THE PREPARATION AND CHARACTERIZATION OF POLY(LACTIDE-CO-GLYCOLIDE) MICROPARTICLES .2. THE ENTRAPMENT OF A MODEL PROTEIN USING A (WATER-IN-OIL)-IN-WATER EMULSION SOLVENT EVAPORATION TECHNIQUE

被引：328

作者：

JEFFERY, H ^{[1
]}

DAVIS, SS ^{[1
]}

OHAGAN, DT ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,NOTTINGHAM NG7 2RD,ENGLAND

来源：

PHARMACEUTICAL RESEARCH | 1993年 / 10卷 / 03期

关键词：

POLY(LACTIDE-CO-GLYCOLIDE); BIODEGRADABLE MICROPARTICLES; (WATER-IN-OIL)-IN-WATER EMULSION; SOLVENT EVAPORATION; CONTROLLED-RELEASE VACCINE; OVALBUMIN;

D O I：

10.1023/A:1018980020506

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Poly(lactide-co-glycolide) (PLG) microparticles with entrapped antigens have recently been investigated as controlled-release vaccines. This paper describes the preparation of PLG microparticles with an entrapped model antigen, ovalbumin (OVA), using a (water-in-oil)-in-water emulsion solvent evaporation technique. In a series of experiments, the effects of process parameters on particle size and OVA entrapment were investigated. It was found that smooth, spherical microparticles 1-2 mum in diameter containing up to 10% (w/w) OVA could be produced using a small volume of external aqueous phase containing a high concentration of emulsion stabilizer and a 1:5 antigen:polymer ratio. PAGE analysis, isoelectric focusing, and Western blotting of OVA released from the microparticles in vitro confirmed that the molecular weight and antigenicity of the protein remained largely unaltered by the entrapment procedure.

引用

页码：362 / 368

页数：7

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