FLUOROMETRIC DNA ASSAY FOR CELL-GROWTH ESTIMATION

被引：198

作者：

RAO, J ^{[1
]}

OTTO, WR ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 207卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90521-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures. © 1992.

引用

页码：186 / 192

页数：7

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