FLUOROMETRIC DNA ASSAY FOR CELL-GROWTH ESTIMATION

被引：198
作者：
RAO, J [1 ]
OTTO, WR [1 ]
机构：
[1] IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 207卷 / 01期
关键词：
D O I：
10.1016/0003-2697(92)90521-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures. © 1992.
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页码：186 / 192
页数：7
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