EFFECTS OF DEXAMETHASONE ON HUMAN SYNOVIAL FIBROBLAST-LIKE CELLS, FROM OSTEOARTHRITIC JOINTS, IN CULTURE

被引：5
|
作者：
VENTO, R
TORREGROSSA, MV
GIULIANO, M
GRECOMORO, G
PICCIONE, F
机构：
[1] UNIV PALERMO,INST HYG,I-90134 PALERMO,ITALY
[2] UNIV PALERMO,INST ORTHOPED,I-90134 PALERMO,ITALY
来源：
LIFE SCIENCES | 1990年 / 47卷 / 24期
关键词：
D O I：
10.1016/0024-3205(90)90150-P
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The effect of Dexamethasone (DEX) on cell division and macromolecular synthesis was investigated in a line (Mc Coy cells, A 9) of synovial fibroblast-like cells derived from human osteoarthritic joints. DEX markedly reduced the proliferation of Mc Coy cells in a time and dose-dependent manner. The maxiamal inhibition (45 %) was found at 500 nM DEX 24 h after incubation and was accompanied by the appearance of giant macrophage-like cells. After DEX treatment cells showed increased content of DNA, proteins and RNA together with the reduction of [3H]-thymidine incorporation into the TCA-precipitable fraction. © 1990.
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收藏
页码：2199 / 2205
页数：7
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