DETECTION OF A GLYCOSYLATED FORM OF HEN EGG-WHITE LYSOZYME

被引：8
作者：
TRUDEL, J [1 ]
ASSELIN, A [1 ]
机构：
[1] UNIV LAVAL,FAC SCI AGR & ALIMENTAT,DEPT PHYTOL,ST FOY,PQ G1K 7P4,CANADA
来源：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1995年 / 73卷 / 5-6期
关键词：
LYSOZYME; GLYCOSYLATION; SEQUENTIAL PAGE; N-TERMINUS MICROSEQUENCING;
D O I：
10.1139/o95-038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By assaying lysozyme activity after denaturing polyacrylamide gel electrophoresis of commercial hen egg white lysozyme preparations, minor lysozymal activity was detected as an 18-kDa protein. After electrophoretic purification for microsequencing, the N-terminus sequence of the 18-kDa lysozyme was found to be identical with mature 14.4-kDa hen egg white lysozyme. The 18-kDa hen egg white lysozyme was judged to be glycosylated based on a 3.6-kDa decrease in molecular mass after N-glycosidase F treatment, binding to concanavalin A - Sepharose, and staining with periodate - Schiff's reagent. The minor form corresponded to about 0.3% of lyzozyme molecules.
引用
收藏
页码：307 / 309
页数：3
相关论文
共 50 条
  • [31] Quantitative study of crystallization kinetics of hen egg-white lysozyme using magnetic orientation
    Wakayama, NI
    JOURNAL OF CRYSTAL GROWTH, 1998, 191 (1-2) : 199 - 205
  • [32] In vitro investigation of the effect of mesalazine on amyloid fibril formation of hen egg-white lysozyme and defibrillation lysozyme fibrils
    Faramarzian, Masoumeh
    Bahramikia, Seifollah
    Shasaltaneh, Marzieh Dehghan
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2020, 874
  • [33] Agitation does not induce fibrillation in reduced hen egg-white lysozyme at physiological temperature and pH
    Malik, Ajamaluddin
    Khan, Javed Masood
    Alhomida, Abdullah S. S.
    Ola, Mohammad Shamsul
    Alokail, Majed S. S.
    Khan, Mohd Shahnawaz
    Alenad, Amal M. M.
    Altwaijry, Nojood
    Alafaleq, Nouf Omar
    Odeibat, Hamza
    JOURNAL OF MOLECULAR RECOGNITION, 2023, 36 (06)
  • [34] Calorimetric evidence for a native-like conformation of hen egg-white lysozyme dissolved in glycerol
    Burova, TV
    Grinberg, NV
    Grinberg, VY
    Rariy, RV
    Klibanov, AM
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1478 (02): : 309 - 317
  • [35] Crystallization and evaluation of hen egg-white lysozyme crystals for protein pH titration in the crystalline state
    Iwai, Wakari
    Yagi, Daichi
    Ishikawa, Takuya
    Ohnishi, Yuki
    Tanaka, Ichiro
    Niimura, Nobuo
    JOURNAL OF SYNCHROTRON RADIATION, 2008, 15 : 312 - 315
  • [36] COMPARISON OF STRUCTURES OF DRY AND WET HEN EGG-WHITE LYSOZYME MOLECULE AT 1.8A RESOLUTION
    KACHALOVA, GS
    MOROZOV, VN
    MOROZOVA, TY
    MYACHIN, ET
    VAGIN, AA
    STROKOPYTOV, BV
    NEKRASOV, YV
    FEBS LETTERS, 1991, 284 (01) : 91 - 94
  • [37] AFM observation of the surface morphology and impurity effects on orthorhombic hen egg-white lysozyme crystals
    Matsuzuki, Y
    Kubota, T
    Liu, XY
    Ataka, M
    Takano, KJ
    JOURNAL OF CRYSTAL GROWTH, 2002, 242 (1-2) : 199 - 208
  • [38] Subtilisin-catalyzed religation of proteolyzed hen egg-white lysozyme: Investigation of the role of disulfides
    Vogel, K
    Cook, J
    Chmielewski, J
    CHEMISTRY & BIOLOGY, 1996, 3 (04): : 295 - 299
  • [39] AMIDE HYDROGEN-EXCHANGE IN A HIGHLY DENATURED STATE - HEN EGG-WHITE LYSOZYME IN UREA
    BUCK, M
    RADFORD, SE
    DOBSON, CM
    JOURNAL OF MOLECULAR BIOLOGY, 1994, 237 (03) : 247 - 254
  • [40] RAMAN-SPECTROSCOPIC STUDIES OF HEN EGG-WHITE LYSOZYME AT HIGH-TEMPERATURES AND PRESSURES
    REMMELE, RL
    MCMILLAN, P
    BIEBER, A
    JOURNAL OF PROTEIN CHEMISTRY, 1990, 9 (04): : 475 - 486
12345